Optimization of a Protocol for the Cryopreservation of Sperm in Pellets for the Common Pheasant (Phasianus colchicus mongolicus)

The sperm of each avian species and breed have unique characteristics that render them more or less susceptible to the freezing–thawing process; therefore, a suitable cryopreservation protocol is specific for type bird needed. In this context, little information about common pheasant’s available. Therefore, aim study was test different parameters at step process freezing into pellets thawing detect least deleterious parameter settings. Sixteen protocols were tested by studying two levels in four steps (dilution, equilibration 5 °C, final dimethylacetamide concentration, time) comprising process. pheasant exhibited high susceptibility damage caused pellets; however, survival reached 29%, greatest recovered mobility 22%. affected dilution viability °C equilibration. found be those with higher rates, 10 min 6% equilibrated 1 min. present study, we individualise some applicable certain critical further investigations are needed order improve upon complete sperm.